Sunday, May 20, 2012
Plasmid DNA preparation                            Plasmid DNA Preparation 

For high throughput or large scale plasmid DNA preparation, we offer molecular biology grade or ultra-low endotoxin grade from both high-copy and low-copy plasmid grown from bacterial or yeast cultures. The standard molecular biology grade plasmids can be used for most downstream applications, such as automated sequencing, restriction enzyme analysis, labeling, ligation, cloning, and PCR without further purification. The ultra-low endotoxin grade plasmids can be used for mammalian cell transfection, in vitro transfection and gene therapy studies.

The Omega Advantage:

  • Flexible Scale: 100 µg to 1000 mg +
  • Predominately supercoil
  • Prepared from high or low-copy plasmids
  • Excellent quality control
  • Endotoxin-free preparation
  • Plasmid prep from yeasts or Agrobacteria available

Customer Provides:

  • Bacterial strain agar plate or glycerol stock 
  • Pelleted cells 
  • Plasmid DNA* 

Turn-around Time:
5-15 days; allow up to 4 weeks extra for large scale endotoxin-free preparations


Product Description Price
 96-well Plasmid Prep 
 1 x 96 Preparations from pelleted cells 
$1.80 / well 
 96-well Plasmid Prep, with growth
 1 x 96 Preparations from 1.2 mL of LB Medium   $2.40 / well 
 96-well Plasmid Prep, Endotoxin-free 
 1 x 96 Preparations from pelleted cells
$2.04 / well 
 96-well Plasmid Prep, Endotoxin-free, with growth
 1 x 96 Preparations from 1.2 mL of LB Medium  
$2.64 / well
 
 
 Plasmid Prep, High-copy** (5 mg)  Preparation of 5 mg DNA from high-copy plasmid  $220
 Plasmid Prep, High-copy (50 mg)  Preparation of 50 mg DNA from high-copy plasmid  $1,200
 Plasmid Prep, High-copy (100 mg)  Preparation of 100 mg DNA from high-copy plasmid  $2,280
     
 Plasmid Prep, Low-copy** (5 mg)  Preparation of 5 mg DNA from low copy plasmid  $480
 Plasmid Prep, Low-copy (50 mg)  Preparation of 50 mg DNA from low-copy plasmid  $2,400
 Plasmid Prep, Low-copy (100 mg)  Preparation of 100 mg DNA from low-copy plasmid  $4,560
     
 Endotoxin-free Grade*** High-copy (5mg)  Endotoxin specification <0.1 EU/mg for 5 mg  $312
 Endotoxin-free Grade High-copy (50mg)  Endotoxin specification <0.1 EU/mg for 50 mg  $1,584
 Endotoxin-free Grade High-copy (100mg)  Endotoxin specification <0.1 EU/mg for 100 mg  $3,000
     
 Plasmid Prep Service Customized  For prep >100 mg, or from yeasts or Agrobacteria  Request a Quote

* Transformation setup fee applies. 
** Yield depends on the characteristics of individual plasmid. Typically high-copy plasmid >3 mg/L of LB; Low-copy plasmid 1~3 mg/L of LB.
*** Omega Bioservices will use an endo-free kit in the prep process; Omega Bioservices does not quantify the endotoxin level in the final product.

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