Southern Blotting Service
 
Invented almost four decades ago, Southern blot is a method for detection of a specific DNA sequence in DNA samples. Genomic DNA is digested with restriction enzyme(s), separated by electrophoresis, then transferred to a filter membrane and subsequent fragment detected by probe hybridization.

Although techniques such as real-time PCR, microarrays and now next generation sequencing may sometimes be used to address the same question, Southern blots performed with restriction enzyme-digested genomic DNA remains the most unequivocal assay to determine the gene copy number in a genome. This is particularly useful in the area of transgenic animals and plants where determining the copy number of transgene is essential. 


The Omega Advantage:

• Flexible: from high throughput 96-well screening blot to confirmatory mini blot
• Seamless integration option: from DNA extraction to Southern blot
• Utilize our proven kit chemistry for maximal DNA integrity and digestibility when bundled with DNA extraction  
  
  

Pricing:

Service	Price
Probe Development / Labeling	$250
96-well Screening Blot	$1500
Confirmatory Mini Blot (up to 18 samples)	$750

   
 
 Turn-around Time:

• Standard 5-day
• Large scale projects please inquire 

Additional Services:

• Confirmation of transgene by PCR
• Copy number assay by quantitative real-time PCR