Tissue | Cell DNA and RNA Extraction Service

Serving the transgenic animal industry and core facilities for years, we have developed a highly efficient program for tissue or cultured cell DNA/RNA extraction. We understand the timing and cost associated with animal maintenance, therefore strive to provide fastest turn around and most accurate extraction and genotyping services to the community. Our kits and robotic instrumentation are configured specifically for high throughput processing of large number of samples. Commonly extracted samples include mouse or rat tail snips, ear punches, distal toe clips, livestock ear punches, hair follicles, fish fin clips, although we can customize our chemistry so any other sample types can be processed efficiently. It is also important to note that we have the option to maximally recover total nucleic acids for a sample, including both genomic and viral DNA and RNA. This enables not only standard genotyping, but also quantitative detection of viruses and bacteria.

The DNA yield varies and is dependent on many factors such as the age of the animal and type of tissue used. Below is just some guideline.

Source	Sample Size	Yield (µg)
Mouse Tail DNA	0.5 - 1 cm snip	15 - 25
Fish Fin DNA	6 mm punch	11 - 18
Mouse Liver DNA	20 mg	13 - 25
HEK 293 Cell DNA	1 x 106	5 - 6
HEK 293 Cell RNA	1 x 106	15 - 30

 The Omega Advantage:

• Ability to process all types of samples: tails, ears, fins, organs, hair, cells
• Ability to extract all nucleic acids: genomic and pathogen
• Automated robotic platforms for all extractions
• NanoDrop quantitation and quality analysis included with no extra charge
• Free cryostorage and requisition for 1 year      

Pricing:

Extraction Service	1-100	101-500	501-1000	1000+
Tissue or Cell DNA	$7.50	$7.09	$6.45	Inquire
Cell RNA	$10.00	$9.45	$8.60	Inquire

   
 
 Turn-around Time:

• Same day service available
• Standard 3-day
• Large scale projects please inquire 

Additional Services:

• Agarose gel electrophoresis QC (DNA/RNA)
• Agilent Bioanalyzer QC (RNA)
• Genotyping: SNP, Fragment Analysis, Sequencing, Southern Blot (DNA)
• Real-time qPCR (DNA/RNA)
• Sample concentration normalization from tube-to-tube or tube-to-plate (DNA/RNA)
• Consultation for downstream analyses and study design