Sunday, May 20, 2012
FFPE DNA RNA Extraction Service

Formalin-fixed, paraffin-embedded (FFPE) tissue is one of the most widely practiced methods for clinical sample preservation and archiving. While standard for histopathology and microscopic investigation, FFPE samples pose a major challenge for molecular pathologists because nucleic acids are heavily modified and trapped by extensive protein-nucleic acid and protein-protein cross linking.  Due to the valuable nature of the FFPE samples, it is paramount that an extraction protocol yields high recovery of DNA/RNA, while also to avoid extensive degradation so that it is amenable for further analysis. 

As one of the leading manufacturers for FFPE DNA and RNA extraction kits, we have a complete line of tools for extracting FFPE samples. Validated by numerous third-party laboratories, our kit-based chemistry typically recovers 2-3x more DNA/RNA than the competitor kits. 
 
   
 DNA suitable for downstream qPCR, microarray, Next Generation Sequencing 
  
 Sections, slides, core punches 
  
 Scalable  - Few samples to thousands of samples per day  (high throughput automation)

Expected Yield

The yield of FFPE samples is highly variable depending on the age, storage condition, fixation method, tissue cellularity and many other factors. We have processed tens of thousands of samples from very different sources, and typically several micrograms or DNA or RNA can be extracted from a 10 micron section. Quite frequently, we can achieve a yield or more than 50 µg.


The Omega Advantage:

  • Ability to process all types of samples: slides, sections or cores
  • High throughput capacity:  FFPE DNA extraction on our liquid handler robotics
  • NanoDrop quantitation and quality analysis included with no extra charge
  • Free cryostorage and requisition for 1 year      

Pricing:

 FFPE DNA Extraction Service 1-100  101-500  501-1000  1000+ 
 FFPE DNA  $24.20

$22.87

$21.30

Inquire


 
 Turn-around Time:
  • Same day service available
  • Standard 3-day
  • Large scale projects please inquire 

Additional Services:

  • Genotyping or mutation analysis
  • Quantitaion by real-time qPCR
  • Sample concentration normalization from tube-to-tube or tube-to-plate
  • Consultation for downstream analyses and study design 

Expected Yield

The yield of FFPE samples is highly variable depending on the age, storage condition, fixation method, tissue cellularity and many other factors. We have processed tens of thousands of samples from very different sources, and typically several micrograms or DNA or RNA can be extracted from a 10 micron section. Quite frequently, we can achieve a yield or more than 50 µg.


The Omega Advantage:

  • Ability to process all types of samples: slides, sections or cores
  • High throughput capacity:  FFPE RNA extraction on our liquid handler robotics
  • NanoDrop quantitation and quality analysis included with no extra charge
  • Free cryostorage and requisition for 1 year      

Pricing:

 FFPE RNA Extraction Service 1-100  101-500  501-1000  1000+ 
 FFPE RNA  $24.20 $22.87 $21.30

Inquire


 
 Turn-around Time:
  • Same day service available
  • Standard 3-day
  • Large scale projects please inquire 

Additional Services:

  • qPCR gene expression analysis
  • Quantitaion by real-time qPCR
  • Sample concentration normalization from tube-to-tube or tube-to-plate
  • Consultation for downstream analyses and study design 
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